CD15 MicroBeads
Description CD15 MicroBeads were developed for positive selection or depletion of CD15+ cells from human blood or single-cell suspensions from tissues. The CD15 antibody recognizes a 3-fucosyl-N-acetyllactosamine carbohydrate modification in glycolipids or glycoproteins. The CD15 antigen is expressed on neutrophils, eosinophils, and monoblastoid precursor cells of the myeloid lineage but not on basophils and lymphocytes. The antigen is also present on Hodgkin/Reed-Sternberg cells. Applications CD15 MicroBeads are used for the isolation1 or depletion of human eosinophils and neutrophils (basophils are CD15-) from peripheral blood and from tissues such as ovarium carcinoma tissue.2 Furthermore, CD15 MicroBeads can be used for the depletion of myeloid cells from bone marrow. Columns For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.		Figure 1 CD15+ cells were isolated from lysed human peripheral blood using CD15 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD15-FITC. Before separation CD15– cells Enriched CD15+ cells