Whole Blood CD33 MicroBeads ( #130-094-773 )

Whole Blood CD33 MicroBeads

Overview

Whole Blood CD33 MicroBeads have been developed for rapid positive selection of CD33⁺ cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.

Details

Background information
The CD33 antigen is prominently expressed on myeloid progenitor cells and monocytes, whereas low-level expression is observed on peripheral granulocytes, dendritic cells, and tissue macrophages. It is not expressed on lymphocytes, platelets, erythrocytes, and primitive hematopoietic stem cells.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD33 MicroBeads to be subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.

Downstream applications
Whole Blood MicroBeads allow the fast isolation of CD33⁺ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD33⁺ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation.

Columns

autoMACS Columns: autoMACS® Pro Separator (Program Posselwb; 0.25–15 mL sample volume), or autoMACS Separator (Program Posseld2; 0.25–3 mL sample volume)
Whole Blood Column Kit: for 0.25–15 mL sample volume

Figure 1

Separation of a whole blood sample using Whole Blood CD33 MicroBeads and the autoMACS® Pro Separator. Cells were fluorescently stained with CD33-APC, CD15-PE, and CD14-FITC. After cell staining erythrocytes were lysed using the Red Blood Cell Lysis Solution.

A: Before separation

B: Enriched CD33+ cells
Counterstaining with CD15-PE

Enriched CD33+ cells<br> Counterstaining with CD15-PE

C: Enriched CD33+ cells
Counterstaining with CD14-FITC

Enriched CD33+ cells<br> Counterstaining with CD14-FITC