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| Diamond Basophil Isolation Kit |
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| Overview |
| The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs. |
| Details |
Background information
Basophilic granulocytes, also known as basophils, are a rare population of leukocytes with frequencies below 1% in peripheral blood. First described by Paul Ehrlich in 1879, basophils have long been considered as circulating mast cells. They express high-affinity immunoglobulin (Ig) E receptors (FcεRI). Upon receptor cross-linking by binding of antigen-IgE complexes, basophils degranulate and thereby release inflammatory mediators such as histamine and leukotriene. Thus, basophils play a major role in allergy, asthma, and immunity.1-4
Detailed separation procedure
First, non-basophils are magnetically labeled for depletion over a MACS Column. The pre-enriched non-labeled basophils in the flow-through are then positively selected via CD123 MicroBeads.
Downstream applications
- Studies on signal requirements for basophil activation, induction of mediator synthesis and release etc.
- Studies on the involvement of basophils in hypersensitivity reactions
- Studies on cytokine expression by basophils
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| Columns |
| For the first magnetic separation (depletion): LS or autoMACS Columns. For the the second magnetic separation (positive selection): MS or autoMACS Columns |
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| Basophils were isolated from human PBMCs using the Diamond Basophil Isolation Kit and two MS Columns. Cells were fluorescently stained with CD123-PE and CD203c-APC and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| PBMCs before separation |
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| Isolated basophils |
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| Details |
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| Products |
| Diamond Basophil Isolation Kit, human |
- for 2×109 total cells
Download datasheet
130-094-434
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| References |
| 1. Shiratori et al. (2005) J. Immunol. 175: 4441–4449. |
| 2. Sloane et al. (2004) Blood 104: 2832–2839. |
| 3. Falcone et al. (2000) Blood 96: 4028–4038. |
| 4. Karasuyama et al. (2009) Nature Rev. Immunol. 9: 9–13. |
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