miRXploreâ„¢ Microarray Kits for microRNA expression profiling
miRXplore™ Microarray Kits miRNA research Small, non-coding microRNA (miRNA) play key roles in cellular differentiation and proliferation pathways as well as apoptosis. Miltenyi Biotecs high-quality miRXplore™ Microarray Kits offer reliable tools for extensive miRNA expression profiling. Advantages of miRXplore™ Microarrays • Expert coverage of sequences Over 2000 miRNA sequences on the miRXplore™ Microarray, spottet in quadruplicates (fig.1), match the latest release of the miRBase sequence database version 12.0. • One microarray for human, mouse, rat, and viral miRNA sequences The miRXplore™ Microarray encompasses in total more than 2000 mature microRNAs - 872 human - 634 mouse - 427 rat - 130 virus, please inquire for further updates. • High sensitive microRNA profiling down to 0,5 amol • Excellent discrimination of closely related microRNAs • Linear dynamic range of more than 4 orders of magnitude • Sample independent normalization Standard normalization procedures can compensate for biased signal intensities, if a sufficient number of miRNA oligonucleotides show no difference in their respective expression level. The 18 synthetic spike-in control oligonucleotides of miRControl 3 provided in the kits are designed to facilitate sample independent normalization. Each microarray kit contains an extensive control system. Kit components The miRXplore Microarray Kits include 4 or 8 microarrays, the hybridization and washing buffers, the PIQOR™ Navigator software, user manual, a configuration overview, and an annotation file. The latter links each microRNA present on the microarray to microRNA-relevant databases, such as the miRBase sequence database. Consistent data Consistant data For reliable microRNA expression profiling 1–5 µg of total RNA is needed. Extensive controls system Controls included in kits: • 13 negative mismatch controls • 36 positive controls (e.g. 5.1 sRNA, tRNA, and U6 RNA) • 5 hybridization controls • 18 calibration controls All control RNA sequences are synthetically generated and are not present in the human, mouse, rat or viral genome, ensuring no cross hybridization. Excellent specificity The miRXplore Microarray enables a highly specific distinction of microRNA sequence variants differing by even only one nucleotide. Closely related microRNAs such as the miR-465 family members show substantial sequence homologies. Using miRXplore Microarrays, the individual member of the miR-465 family can be easily distinguished. A variety of miRNAs show tissue-dependent expression patterns. With the miRXplore Microarrays tissue-specific miRNA can be identified. Highly sensitive signal detection The miRXplore Microarray is compatible with conventional glass slide scanners and miRNA quantities down to 0.5 amol can be detected linearily offering a dynamic range of more than 4 orders of magnitude. Microarray production The miRXplore Microarrays are accurately produced under stringent quality control to enable valid and reliable expression profiling. All microRNA sequences are spotted in quadruplicates, thus further enhancing signal data robustness. Comprehensive miRXplore Services Miltenyi Biotec offers a comprehensive, cost-effective and fast miRNA expression profiling service. Read more.. Gene expression profiling from one single cell You have less sample material and want to do gene expression profiling? With our SuperAmp™ Service we amplify your RNA even from one single cell. Use this service in combination with our PIQOR™ and Agilent Microarray Services. Please note that the SuperAmp Service is not applicable to microRNA amplification. Further information Average RNA yields [PDF; 87,7 KB] miRXplore™ Microarray flyer [PDF; 333,8 KB]		Figure 1 Overview miRXplore™ Microarray Position of control RNA miRControl 1 on each microarray is shown. The oligonucleotide sequences on the miRXplore™ Microarrays are complementary to the respective mature miRNAs. All sequences on the miRXplore Microarrays are spotted in quadruplicates. Figure 2 Highly reproducible results. Total RNA was isolated from human CD4+ T cells, and aliquots of 5 μg and 10 μg were labeled with Cy5 fluorescent dye. The miRXplore™ Universal Reference, a synthetic miRNA pool, was labeled with Cy3 fluorescent dye. Labeled Universal Reference was hybridized together with either the 5 μg or the 10 μg labeled RNA sample to miRXplore Microarray 1 and 2, respectively. The scatter plot shows the log ratios of signal intensities of the 5 μg–sample/pool (microarray 1) versus the 10 μg–sample/pool (microarray 2). The high Pearson correlation coefficient (r = 0.99) demonstrates near-identical signals on both microarrays. Figure 3 Probe-target specificity of miRXplore™ Microarray. 5 μg of human total RNA from hepatocellular carcinoma cells, glioblastoma cells, hippocampus cells, and CD4+ T cells were individually fluorescently labeled and hybridized to four miRXplore™ Microarrays. The figure illustrates the relative signal intensities of the single-mismatch (mut 1) and double-mismatch (mut 2) probes, with the perfect-match signal intensities set to 100%. Figure 4 Easy detection of miRNA family members Closely related miRNAs such as the miR-465 family members show substantial sequence homologies. Using miRXplore Microarrays, the individual member of the miR-465 family can be easily distinguished. Figure 5 Accurate tissue-specific miRNA identification A variety of miRNAs show tissue-dependent expression patterns, for example, miR-133a is only detected in heart tissue. In experiments with miRXplore Microarrays, the highly tissue specific expression of miR-133a and other selectively expressed miRNA was confirmed.

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