EasyProbe Transcription Templates, human
IVT templates for the generation of in situ probes Easy preparation of highly specific RNA probes! In situ hybridization (ISH) and northern blot analysis are common and powerful tools for the detection and quantification of mRNA. To achieve reliable and reproducible results, it is essential to use single-stranded RNA probes that have a high sequence specificity. Often, the generation of these high-quality RNA probes turns out to be more tedious and time-consuming than expected. Miltenyi Biotec's sequence-verified EasyProbe Transcription Templates are ready-to-use, linear DNA templates (in vitro transcription templates) for the generation of specific RNA probes for in situ hybridization or northern blot analysis. Two main research topics On this page you will find 47 transcription templates for the generation of RNA probes specific to human skin-related mRNAs1 (fig. 2). Next to this, Miltenyi Biotec offers 39 transcription templates specific to mouse neural mRNAs. Click here for more information on mouse neural-specific EasyProbe Transcription Templates. Or just get a complete overview and download the EasyProbe Gene List to check if an EasyProbe Transcription Template is available for your gene of interest. Principle EasyProbe Transcription Templates are linear, double-stranded DNA templates ready-to-use for in vitro transcription (IVT). Delivered as lyophilizates, the template only needs to be solubilized in water before IVT (fig. 1). Depending on the individual experimental setting, the RNA probes can be generated using diverse labels such as fluorescence (e.g. Cy5 and Cy3), digoxygenine (DIG), biotin, or radioactive isotopes. The gene-specific segment of the templates ranges from 250–400 basepair (bp). The supplied 200 ng lyophilizate will suffice for five IVT reactions producing enough RNA probes for 20–50 in situ hybridizations experiments or northern blot analyses. Download data sheet for more detailed information. Applications In situ hybridization Northern blot Further information Data sheet Easyprobe Transcription Templates, human, mouse [PDF; 284,2 KB]		Figure 1 Principle of in situ probe generation with EasyProbe Transcription Templates Figure 2 In situ hybridization of interferon α in human skin Lichen planus lesions. Digoxygenin-labeled probes were generated using an EasyProbe Transcription Template. Upper picture: detection of interferon α. Lower picture: control.

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