CD154
Description This antibody recognizes the human CD154 antigen, a transmembrane glycoprotein also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4+ T cells and plays an important role as a costimulatory molecule in T cell/antigen-presenting cell interactions through ligation of CD40. Due to its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4+ T cells. Adding a CD40-blocking antibody during the stimulation of cell suspensions prevents down-regulation of CD154 expression induced by interaction with CD40 expressed on antigen-presenting cells. This monoclonal antibody was shown to cross-react with rhesus monkey (Macaca mulatta) and cynomolgus monkey (Macaca fascicularis) cells. Applications The CD154 antibodies are suitable to identify and enumerate activated antigen-specific CD4+ T cells by flow cytometry or fluorescence microscopy. Other applications are evaluation of intracellular cytokine expression in activated antigen-specific CD4+ T cells by using CD154 antibodies in combination with antibodies against cytokines. Clone Isotype 5C8 Mouse IgG2a		Figure 1 Rhesus monkey PBMCs were stimulated using the T Cell Activation/Expansion Kit, non-human primate. Cells were stained with CD154-PE and CD4-APC and analyzed by flow cytometry. Stimulated sample Unstimulated control Figure 2 Cynomolgus monkey PBMCs were stimulated using the T Cell Activation/Expansion Kit, non-human primate. Cells were stained with CD154-PE and CD4-APC and analyzed by flow cytometry. Stimulated sample Unstimulated control

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