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| Description |
Clone DX2 specifically recognizes human CD95 (also termed Fas and Apo-1) but cross-reacts with rhesus monkey (Macaca mulatta) and cynomolgus monkey (Macaca fascicularis) CD95. CD95 is a member of the tumor necrosis factor receptor superfamily (TNFR), found on the surface of many normal and neoplastically transformed cells. Its ligand, CD95L (FasL/Apo-1L), is able to induce apoptosis in CD95-expressing cells upon binding1. CD95 and CD95L are up-regulated on lymphocytes upon activation and are known to play a key role in the regulation of an inflammatory response: juxtocrine “fratricide” of neighbouring lymphocytes via mutual CD95 and CD95L expression helps to terminate immune responses2, while apoptosis of pro-inflammatory cells via CD95 helps maintain immune privilege in sites such as the eye, where CD95L is found to be expressed in the retina and cornea3. Cross-linking of CD95 receptors by DX2 monoclonal antibody has been described to induce apoptosis in certain target cells.4
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| Applications |
| Identification and enumeration of CD95+ cells by flow cytometry and fluorescence microscopy. |
| Clone | Isotype |
| DX2 | Mouse IgG1 |
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| Figure 1 |
| Rhesus monkey (A) or cynomolgus monkey (B) PBMCs were stained with CD95-PE and CD3-APC, or CD95-FITC and CD3-APC, respectively, and analyzed by flow cytometry. |
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| B |
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| References |
| 1. Itoh et al. (1991) Cell 66: 233–243. |
| 2. Lynch et al. (1995) Immunol. Today 16: 569–574. |
| 3. Griffith et al. (1995) Science 270: 1189–1192. |
| 4. Komada, Y. et al. (1999) Hybridoma 18: 391–398. |
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