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| Description |
Clone 4E3 is specific for human CD25 but also cross-reacts with with CD25 of rhesus monkey (Macaca mulatta) and cynomolgus monkey (Macaca fascicularis) cells. CD25 is a 55 kDa glycoprotein and is also known as the low-affinity interleukin 2 receptor alpha chain (IL-2Rα). CD25 is expressed on human activated T and B cells and macrophages. CD25 is also present on a subset of non-activated CD4+ T cells which act as regulatory T cells.
The CD25 antigen contains three epitope regions called A, B, and C. Clone 4E3 recognizes epitope region B. |
| Applications |
| The CD25 antibody can be used to identify and enumerate cells by flow cytometry or fluorescence microscopy. This antibody is highly suitable for MACS® Control applications after using CD25 MicroBeads. The CD25 antibody recognizes a different epitope (epitope B) than the CD25 MicroBeads (epitope A). For further details, please refer to the data sheet of the separation reagent. |
| Clone | Isotype |
| 4E3 | Mouse IgG2b |
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| Figure 1 |
| Rhesus monkey PBMCs were stained with CD25-PE and CD4-APC. |
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| Figure 2 |
Cynomolgus monkey peripheral blood cells were stained with CD25-PE and CD4-APC and analyzed by flow cytometry.
(Data were kindly provided by the California National Primate Research Center, Davis, CA, USA.) |
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| Products |
| CD25-PE, human |
- for 100 tests (1)
Download data sheet
130-091-024
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| CD25-APC, human |
- for 100 tests (1)
Download data sheet
130-092-858
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| CD25-Biotin, human |
- for 100 tests (2)
Download data sheet
130-091-235
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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