| Description |
| Interleukin 2 (IL-2), mainly produced by activated CD4+ T cells, is a central regulator of the immune response. It induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, monocytes. and oligodendrocytes. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance, and anti-inflammatory reactions. |
| Applications |
| This Anti-IL-2 antibody has been developed for intracellular staining of IL-2 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separations. |
| Clone | Isotype |
| JES6-5H4 | Rat IgG2b |
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| Figure 1 |
| Mouse spleen cells were incubated with or without KLH for 6 hours. After 2 hours, brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-2-PE and CD154-APC. Cells were analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells. |
| a: Stimulated sample |
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| b: Unstimulated control |
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