Anti-TNF-α
Description Mouse tumor necrosis factor α (TNF-α) is secreted by macrophages, monocytes, neutrophils, CD4+ T cells, and NK cells. It has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Applications This Anti-TNF-α antibody has been developed for intracellular staining of TNF-α in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separations. Clone Isotype MP6-XT22 Rat IgG1		Figure 1 Mouse spleen cells were incubated with or without KLH for 6 hours. After 2 hours, brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-TNF-α-PE and CD154-APC. Cells were analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells. Stimulated sample Unstimulated control

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