| Description |
| Mouse interferon gamma (IFN-γ) is produced by activated T, B, and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. |
| Applications |
| This Anti-IFN-γ antibody has been developed for intracellular staining of IFN-γ in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separations. |
| Clone | Isotype |
| AN18.17.24 | Rat IgG |
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| Figure 1 |
| Mouse spleen cells were incubated with or without KLH for 6 hours. After 2 hours, brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE and CD154-APC. Cells were analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells. |
| Stimulated sample |
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| Unstimulated control |
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