Anti-IL-17
Description The Anti-IL-17 antibodies are designed for intracellular staining of IL-17A–producing cells. Interleukin 17 (IL-17) is a family of cytokines that play a central role in adaptive immunity as well as autoinflammatory disorders.1 Data from several mouse models suggest that IL-17 plays a key role in the host defense against certain extracellular bacterial infections. Recently, a new mouse T helper cell subset has been identified, which is dinstinct from Th1 and Th2 cells: IL-17–producing CD4+ T helper (Th17) cells. Th17 cells preferentially produce IL-17A, IL-17F, IL-21, and IL-22. Receptors for IL-17 and IL-22 are expressed on various epithelial tissues, thus Th17 cells are crucial for the cross-talk between immune system and tissues.2 It is now established that Th17 cells are responsible for driving autoimmune inflammation. After stimulation of mouse T cells, IL-17 is not only secreted by CD4+ T cells, but also by CD8+ T cells and γδ T cells. Applications This Anti-IL-17 antibody has been developed for intracellular staining of IL-17 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by in-column intracellular staining technology in combination with MACS® Separation. Clone Isotype TC11-18H10 Rat IgG1		Figure Mouse spleen cells were incubated with or without PMA/ionomycin for 6 hours. After 2 hours brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-17-PE. Cell surface staining was performed with CD4-APC. Cells were analyzed by flow cytometry. B cells and cell debris were excluded from the analysis. a: Stimulated sample b: Unstimulated control

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