|
|
 |
|
| Description |
The Anti-PSA-NCAM antibody recognizes an epitope on human, mouse, and rat polysialic acid (PSA) which, in vertebrates, is found linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56)1. PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue.2 In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ)3 and the dentate gyrus of the hippocampus4.
PSA-NCAM is a marker for immature neuronal committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons.3,5
Antibodies against PSA-NCAM have also been used to immunomagnetically isolate neuronal progenitors from postnatal mice and rat forebrain.6–8 |
| Applications |
| Identification and enumeration of human, mouse, and rat PSA-NCAM+ cells by flow cytometry, fluorescence microscopy, or immunohistochemistry in conjunction with a conjugated secondary antibody. |
| Clone | Isotype |
| 2-2B | Mouse IgM |
| |
| Figure 1 |
| Mouse brain tissue postnatal day 1 was dissociated using the Neural Tissue Dissociation Kit (T). Brain cells were stained with Anti-PSA-NCAM-PE (A) or Anti-PSA-NCAM-APC (B). Cells were analyzed by flow cytometry. |
| A |
 |
| B |
 |
|
|
|
|
| My favorites |
| Your current favorite list is empty. |
| Products |
| Anti-PSA-NCAM-PE, human, mouse, rat |
- for 100 tests (1)
Download data sheet
130-093-274
|
| Anti-PSA-NCAM-APC, human, mouse, rat |
- for 100 tests (2)
Download data sheet
130-093-273
|
| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to fluorescent labeling of up to 106 cells in a total volume of 100 µL. |
|
| References |
| 1. Rougon and Marshak (1986) J. Biol. Chem. 261: 3396–3401. |
| 2. Kiss and Muller (2001) Rev. Neurosci. 12: 297–310. |
| 3. Doetsch et al. (1997) J. Neurosci. 17: 5046–5061. |
| 4. Seki (2002) J. Neurosci. Res. 70: 327–334. |
| 5. Pennartz et al. (2004) Mol. Cell. Neurosci. 25: 692–706. |
| 6. Seidenfaden et al. (2006) Mol. Cell. Neurosci. 32: 187–98. |
| 7. Seidenfaden et al. (2006) MACS&more 10(1): 4–6. |
| 8. Marmur et al. (1998) J. Neurosci. 18: 9800–9811. |
|
|