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| Description |
Clone D7 specifically recognizes the mouse Sca-1 antigen. Sca-1 (stem cell antigen-1) is an 18 kDa GPI-linked surface protein of the Ly-6 family (Ly-6A/E). The Anti-Sca-1 antibody recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly6A.2: e.g. C57BL/6, SJL, 129, AKR).
Sca-1 is expressed on stem cells in bone marrow with hematopoietic differentiation potential. Sca-1 is also expressed on a population of CD105+ hematopoietic stem cells (HSCs). This population has a long-term repopulating (LTR) capacity and is therefore termed LTR-HSCs1,2. Sca-1+ cells from bone marrow also possess nonhematopoietic differentiation potential as they are able to give rise to hepatocytes in vivo3 and neural cells in vitro4.
Sca-1 expression is also found on distinct subpopulations of bone marrow and peripheral B cells, myeloid cells, thymic and peripheral lymphocytes, and early intrathymic T cell precursors.
Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver5, heart6, and prostate7. |
| Applications |
| The Anti-Sca-1 antibody can be used to identify and characterize Sca-1+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent. |
| Clone | Isotype |
| D7 | Rat IgG2a |
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| Figure 1 |
| Mouse bone marrow cells were stained with Anti-Sca-1 antibodies conjugated to FITC (A), PE (B), APC (C), or biotin (D) and analyzed by flow cytometry. Cells stained with Anti-Sca-1-Biotin were additionally stained with Anti-Biotin-APC. |
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| B |
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| C |
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| D |
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| Products |
| Anti-Sca-1-FITC, mouse |
- for 100 tests (1)
Download data sheet
130-093-222
|
| Anti-Sca-1-PE, mouse |
- for 100 tests (1)
Download data sheet
130-093-224
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| Anti-Sca-1-APC, mouse |
- for 100 tests (1)
Download data sheet
130-093-223
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| Anti-Sca-1-Biotin, mouse |
- for 100 tests (2)
Download data sheet
130-093-421
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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| References |
| 1. Chen et al. (2002) Proc. Natl. Acad. Sci. USA 99: 15468–15473. |
| 2. Chen et al. (2003) Immunity 19: 525–533. |
| 3. Lagasse et al. (2000) Nat. Med. 6: 1229-1234.[939] |
| 4. Sanchez-Ramos et al. (2000) Exp. Neurol. 164: 247-256. |
| 5. Petersen et al. (2003) Hepatology 37: 632-640.[11259] |
| 6. Matsuura et al. (2004) J. Biol. Chem. 279: 11384-11391.[4144] |
| 7. Burger et al. (2005) Proc. Natl. Acad. Sci. USA 102: 7180-7185.[7082] |
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