CD205 (DEC205)
Description The CD205 antibody recognizes a 205 kDa integral membrane glycoprotein also known as DEC205 (dendritic and epithelial cells, 205 kDa).1,2 This membrane protein acts as an endocytic receptor and thereby mediates efficient processing and presentation of antigens in vivo, leading to the induction of T cell immunity or tolerance3. CD205 is expressed at high levels on mouse dendritic cells (DCs) in the skin (Langerhans cells), on DCs residing in the T cell areas of peripheral lymphoid organs, and on DCs generated in vitro from bone marrow progenitors.4 To a much lower extent, CD205 is also expressed on mature B cells, granulocytes, and T cells.1,2 Applications The CD205 antibodies can be used for the detection or subset specific analysis of dendritic cells in combination with other markers, such as CD11c, CD8, or MHC Class II. They are suitable for the identification and enumeration of CD205+ cells by flow cytometry or fluorescence microscopy. Clone Isotype NLDC-145 Rat IgG2a		Figure 1 Mouse spleen cells were stained with CD8-conjugated antibodies as well as with CD205 (DEC205) antibodies conjugated to PE (A), APC (B), or Biotin (C). Cells stained with CD205 (DEC205)-Biotin were stained with Anti-Biotin-PE in addition. Samples were then analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. A B C

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