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MACSQuant® Analyzer
navigationAntibodies
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Biotec
for human cellsfor human cellsfor human cells
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Biotec
for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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Biotec
for mouse cellsfor mouse cellsfor mouse cells
AntibodiesAntibodiesAntibodiesAntibodies
Cytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodies
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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Biotec
for rat cellsfor rat cellsfor rat cells
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Biotec
for indirect stainingfor indirect stainingfor indirect staining
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Biotec
Cytokine detection
Kits for cell detection and analysis
Tumor cell analysis
Immunofluorescent amplification (FASER Kits)
Inside Stain Kit
Staining of apoptotic and dead cells
Compensation reagents
Antibody biotinylation
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CD146 (LSEC)
Description
The CD146 (LSEC) antibody (clone ME-9F1) recognizes the CD146 antigen, which is expressed on mouse endothelial cells, including liver sinusoidal endothelial cells (LSECs), smooth muscle cells, and the basal membrane.1 LSECs are microvascular endothelial cells lining the hepatic sinusoidal wall. Their strategic positioning favors a tight interaction with lymphocytes migrating through the liver. They possess a high capacity for antigen uptake and processing. However, in contrast to professional antigen-presenting cells (e.g. dendritic cells), they express only low levels of costimulatory molecules.2 LSECs are supposed to mainly contribute to the control of immune responses against circulating soluble antigens in the liver.
Applications
The CD146 (LSEC) antibody can be used to identify and enumerate LSECs or other CD146+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent.
Clone Isotype
ME-9F1Rat IgG2a
 
Figure 1
Mouse liver cells were stained with CD146 (LSEC) antibodies conjugated to FITC (A) or Biotin (B). Cells stained with CD146 (LSEC)-Biotin were also stained with Anti-Biotin-APC and analyzed by flow cytometry.
A
B
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Products
CD146 (LSEC)-FITC, mouse
- for 100 tests (1)
Download datasheet
130-092-026
Qty.:
 

CD146 (LSEC)-Biotin, mouse
- for 100 tests (2)
Download datasheet
130-092-025
Qty.:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL.
(2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL.
References
1. Harder et al. (1991) Exp. Cell Res. 197: 259–267.
2. Diehl et al. (2008) Hepatology 47: 296–305.
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