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CD45R (B220)
Description
Clone RA3-6B2 recognizes the mouse CD45R (B220) antigen which is expressed on B lymphocytes throughout their develop­ment from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R monoclonal antibody clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45. CD45R is absent in thymus but reported to be present on apoptotic thymocytes.
Applications
The CD45R (B220) antibody can be used to identify and enumerate CD45R (B220)+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent.
CloneIsotype
RA3-6B2Rat IgG2a
 
Figure 1
Mouse splenocytes were stained with CD45R (B220) antibodies conjugated to FITC (A), PE (B), APC (C), or VioBlue (D), and analyzed by flow cytometry.
A
B
C
D
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Products
CD45R (B220)-FITC, mouse
for 100 tests (1)
Download data sheet
130-091-829
Qty:
 

CD45R (B220)-PE, mouse
for 100 tests (1)
Download data sheet
130-091-828
Qty:
 

CD45R (B220)-APC, mouse
for 100 tests (1)
Download data sheet
130-091-843
Qty:
 

CD45R (B220)-VioBlue, mouse
for 100 tests (1)
Download data sheet
130-094-287
Qty:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL.
Related products
CD45R (B220) MicroBeads, mouse (#130-049-501)
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