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MACSQuant® Analyzer
navigationAntibodies
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Biotec
for human cellsfor human cellsfor human cells
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Biotec
for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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Biotec
for mouse cellsfor mouse cellsfor mouse cells
AntibodiesAntibodiesAntibodiesAntibodies
Cytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodies
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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for rat cellsfor rat cellsfor rat cells
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Biotec
for indirect stainingfor indirect stainingfor indirect staining
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Biotec
Cytokine detection
Kits for cell detection and analysis
Tumor cell analysis
Immunofluorescent amplification (FASER Kits)
Inside Stain Kit
Staining of apoptotic and dead cells
Compensation reagents
Antibody biotinylation
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CD45
Description
Clone 30F11 recognizes the mouse CD45 antigen (Ly-5, leukocyte common antigen) which is expressed at high levels on all cells of hematopoietic origin except for erythrocytes. CD45 can be used to discriminate leukocytes from nonhematopoietic cells. The CD45 antibody clone 30F11 reacts with all CD45 isoforms.
The VioBlue-conjugated CD45 antibody requires the use of a violet laser.
Applications
The CD45 antibody can be used to identify and enumerate CD45+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable to evaluate MACS® Separations. For further details, please refer to the data sheet of the separation reagent.
Clone Isotype
30F11Rat IgG2b
 
Figure 1
Mouse splenocytes were stained with CD45 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (D) and analyzed by flow cytometry using the MACSQuant® Analyzer..
A
B
C
D
E
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Products
CD45-FITC, mouse
- for 100 tests (1)
Download datasheet
130-091-609
Qty.:
 

CD45-PE, mouse
- for 100 tests (1)
Download datasheet
130-091-610
Qty.:
 

CD45-APC, mouse
- for 100 tests (1)
Download datasheet
130-091-811
Qty.:
 

CD45-VioBlue, mouse
- for 100 tests (1)
Download datasheet
130-092-910
Qty.:
 

CD45-PerCP, mouse
- for 100 tests (1)
Download datasheet
130-094-962
Qty.:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL.
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