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| Description |
Clone 30F11 recognizes the mouse CD45 antigen (Ly-5, leukocyte common antigen) which is expressed at high levels on all cells of hematopoietic origin except for erythrocytes. CD45 can be used to discriminate leukocytes from nonhematopoietic cells. The CD45 antibody clone 30F11 reacts with all CD45 isoforms.
The VioBlue-conjugated CD45 antibody requires the use of a violet laser. |
| Applications |
| The CD45 antibody can be used to identify and enumerate CD45+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable to evaluate MACS® Separations. For further details, please refer to the data sheet of the separation reagent. |
| Clone | Isotype |
| 30F11 | Rat IgG2b |
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| Figure 1 |
| Mouse splenocytes were stained with CD45 antibodies conjugated to FITC (A), PE (B), APC (C), or VioBlue (D) and analyzed by flow cytometry. |
| A |
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| B |
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| C |
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| D |
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