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CD11c
Description
The CD11c antibody clone N418 reacts with the αx subunit of the leukocyte integrin gp150/95 (CD11c/CD18). The mouse CD11c antigen is present on dendritic cells in lymphoid organs and blood, on Langerhans cells in the epidermis, on dendritic cell progenitors in the bone marrow, and on in vitro generated bone marrow-derived dendritic cells. In spleen and lymph node, CD11c is expressed at high levels on conventional CD11c+CD45R–mPDCA-1– dendritic cells, and at moderate levels on CD11c+CD45R+ mPDCA-1+ plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, and <1% of lymph node cells and thymocytes express CD11c.
Applications
The CD11c antibody can be used to identify and enumerate CD11c+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent.
Clone Isotype
N418Hamster IgG
 
Figure 1
Mouse splenocytes were stained with CD11c antibodies conjugated to FITC (A), PE (B), or APC (C) and Anti-MHC Class II-PE or -FITC. The samples were then analyzed by flow cytometry.
A
B
C
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Products
CD11c-FITC, mouse
- for 100 tests (1)
Download data sheet
130-091-842
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CD11c-PE, mouse
- for 100 tests (1)
Download data sheet
130-091-830
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CD11c-APC, mouse
- for 100 tests (1)
Download data sheet
130-091-844
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(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL.
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