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Anti-MHC Class II
Description
Clone M5/114.15.2 recognizes mouse major histocompatibility complex (MHC) Class II antigen. MHC Class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes/macrophages, and on B cells in lymphoid and non-lymphoid tissue, on thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.
The monoclonal Anti-MHC Class II antibody reacts with the MHC Class II alloantigens I-Ab, I-Aq, I-Ad, I-Ed, and I-Ek that are expressed by most common inbred mouse strains, for example, C57BL/6, BALB/c, or 129/SvEv.
Applications
The Anti-MHC Class II antibody can be used to identify and enumerate MHC Class II+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent.
Clone Isotype
M5/114.15.2Rat IgG2b
 
Figure 1
Mouse splenocytes were stained with Anti-MHC Class II antibodies conjugated to FITC (A), PE (B), or APC (C) and analyzed by flow cytometry.
A
B
C
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Products
Anti-MHC Class II-FITC, mouse
- for 100 tests (1)
Download data sheet
130-081-601
Qty:
 

Anti-MHC Class II-PE, mouse
- for 100 tests (1)
Download data sheet
130-091-368
Qty:
 

Anti-MHC Class II-APC, mouse
- for 100 tests (1)
Download data sheet
130-091-806
Qty:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL.
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