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| Description |
Clone M5/114.15.2 recognizes mouse major histocompatibility complex (MHC) Class II antigen. MHC Class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes/macrophages, and on B cells in lymphoid and non-lymphoid tissue, on thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow. The monoclonal Anti-MHC Class II antibody reacts with the MHC Class II alloantigens I-Ab, I-Aq, I-Ad, I-Ed, and I-Ek that are expressed by most common inbred mouse strains, for example, C57BL/6, BALB/c, or 129/SvEv. |
| Applications |
| The Anti-MHC Class II antibody can be used to identify and enumerate MHC Class II+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent. |
| Clone | Isotype |
| M5/114.15.2 | Rat IgG2b |
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| Figure 1 |
| Mouse splenocytes were stained with Anti-MHC Class II antibodies conjugated to FITC (A), PE (B), or APC (C) and analyzed by flow cytometry. |
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| B |
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| C |
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| Products |
| Anti-MHC Class II-FITC, mouse |
- for 100 tests (1) Download data sheet 130-081-601
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| Anti-MHC Class II-PE, mouse |
- for 100 tests (1) Download data sheet 130-091-368
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| Anti-MHC Class II-APC, mouse |
- for 100 tests (1) Download data sheet 130-091-806
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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