Anti-H-2Kk ( #130-085-101 )

Anti-H-2K^k

Description

Clone H100-27.R55 specifically recognizes the mouse H-2K^k antigen, an MHC Class I alloantigen expressed in mice of an H-2K^k haplotype as well as cells transfected with pMACS K^k.II using the MACSelect™ K^k - Transfected Cell Selection Kits.

Applications

The antibody can be used to identify and enumerate mouse MHC class I⁺ cells of H-2K^k haplotype and to evaluate cells transfected with pMACS K^k.II using MACSelect™ K^k-Transfected Cell Selection Kits. To control the efficiency of transfection, transfected cells are fluorescently stained with Anti-H-2K^k-FITC and analyzed by flow cytometry or fluorescence microscopy.

For details regarding the MACSelect™ System, click here.

Clone	Isotype
H100-27.R55	Mouse IgG2a

Figure 1

Cells from the mouse pre–B cell 1881 cell line were stably transfected with the H-2K^kgene and mixed with untransfected cells from this cell line in a 1:3 ratio. The mixed cells were stained with Anti-H-2K^k-FITC and analyzed by flow cytometry.