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MACSQuant® Analyzer
navigationAntibodies
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Biotec
for human cellsfor human cellsfor human cells
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Biotec
for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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Biotec
for mouse cellsfor mouse cellsfor mouse cells
AntibodiesAntibodiesAntibodiesAntibodies
Cytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodies
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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Biotec
for rat cellsfor rat cellsfor rat cells
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Biotec
for indirect stainingfor indirect stainingfor indirect staining
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Biotec
Cytokine detection
Kits for cell detection and analysis
Tumor cell analysis
Immunofluorescent amplification (FASER Kits)
Inside Stain Kit
Staining of apoptotic and dead cells
Compensation reagents
Antibody biotinylation
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CD25
Description
The CD25 antibody recognizes the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4+CD25+ regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor.
Applications
CD25 is suitable for staining of activated T and B cells. In combination with CD4, it can be used to identify regulatory T cells (Tregs).
Clone Isotype
7D4Rat IgM
 
Figure 1
Mouse splenocytes were stained with CD25-PE (A) or CD25-Biotin and Anti-Biotin-PE (B) and CD4-FITC and analyzed by flow cytometry.
A
B
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Products
CD25-PE, mouse
- for 100 tests (1)
Download datasheet
130-091-013
Qty.:
 

CD25-APC, mouse
- for 100 tests (1)
Download datasheet
130-093-734
Qty.:
 

CD25-Biotin, mouse
- for 100 tests (2)
Download datasheet
130-092-569
Qty.:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL.
(2) One tests corresponds to labeling of up to 107 cell in a total volume of 100 µL.
Related products
CD4+CD25+ Regulatory T Cell Isolation Kit, mouse (#130-091-041)
CD25 MicroBead Kit, mouse (#130-091-072)
CD4 antibodies
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