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| Description |
Interleukin 12 (IL-12) is a 70–75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is mainly produced by monocytes/macrophages and dendritic cells, primarily in response to stimulation with bacterial or viral antigens.
The Anti-IL-12 (p35/p70) antibody reacts with both, the p35 chain and the p70 heterodimer of human IL-12. |
| Applications |
| This Anti-IL-12 (p35/p70) antibody has been developed for intracellular staining of IL-12 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by in-column intracellular staining technology in combination with MACS® Separation. |
| Clone | Isotype |
| B-T21 | Mouse IgG1 |
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| Figure |
| Human PBMCs were stimulated with LPS/ R848 for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-12 (p35/p70) antibodies conjugated to PE (A) or APC (B). Cells were analyzed by flow cytometry. |
| A |
| Stimulated sample |
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| Unstimulated control |
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| B |
| Stimulated sample |
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| Unstimulated control |
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| Products |
| Anti-IL-12 (p35/p70)-PE, human |
- for 100 tests (1)
Download data sheet
130-093-432
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| Anti-IL-12 (p35/p70)-APC, human |
- for 100 tests (1)
Download data sheet
130-093-431
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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