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| Description |
Interleukin 12 (IL-12) is a 70-75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is produced mainly by monocytes/macrophages and dendritic cells, primarily in response to stimulation with bacterial or viral antigens. IL-12 displays various effects on both T and NK cells, playing an essential role in the interaction between innate and adaptive immunity.
Clone 8.6 reacts with both, the p40 chain and the p70 heterodimer of human IL-12. |
| Applications |
| This Anti-IL-12 antibody has been developed for intracellular staining of IL-12 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separation. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| 8.6 | Mouse IgG1 |
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| Figure 1 |
| Human PBMCs were stimulated with LPS / R848 for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-12 (p40/p70)-PE. Cells were analyzed by flow cytometry. |
| A: Stimulated sample |
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| B: Unstimulated control |
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| Products |
| Anti-IL-12 (p40/p70)-PE, human |
For 100 tests (1)
Download data sheet
130-092-774
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| Anti-IL-12 (p40/p70)-APC, human |
For 100 tests (1)
Download data sheet
130-092-775
|
| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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