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| Description |
| Interleukin-10 (IL-10) is predominantly secreted by human CD4+ T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by TH1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells. |
| Applications |
| This Anti-IL-10 antibody has been developed for intracellular staining of IL-10 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separation. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| B-T10 | Mouse IgG1 |
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| Figure 1 |
| Human PBMCs were incubated with or without CytoStim for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-10-PE. Cells were then analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-APC staining. |
| Stimulated sample |
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| Unstimulated control |
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| Products |
| Anti-IL-10-PE, human |
- for 100 tests (1)
Download data sheet
130-091-643
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| Anti-IL-10-APC, human |
- for 100 tests (1)
Download data sheet
130-091-642
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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