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| Description |
| Interleukin-5 (IL-5) is predominantly secreted by CD4+ T cells. It promotes growth and activation of eosinophils. Furthermore, it is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells. |
| Applications |
| This Anti-IL-5 antibody has been developed for intracellular staining of IL-5 in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separation. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| JES1-39D10 | Rat IgG2a |
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| Figure 1 |
| Human PBMCs were incubated with or without PMA/ionomycin for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-5-PE. Cells were then analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-APC staining. |
| Stimulated sample |
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| Unstimulated control |
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| Products |
| Anti-IL-5-PE, human |
for 100 tests (1)
Download data sheet
130-091-648
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| Anti-IL-5-APC, human |
for 100 tests (1)
Download data sheet
130-091-834
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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