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| Description |
| Tumor necrosis factor α (TNF-α) is produced by cells that are involved in inflammatory immune responses. TNF-α is secreted by activated CD4+ T cells, monocytes, macrophages, NK cells, and neutrophils. |
| Applications |
| This Anti-TNF-α antibody has been developed for intracellular staining of TNF-α in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separation. |
| Clone | Isotype |
| cA2 | Human IgG1 |
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| Figure 1 |
| Human PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-TNF-α-PE. Cells were analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-FITC staining. |
| Stimulated sample |
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| Unstimulated control |
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| Products |
| Anti-TNF-α-FITC, human |
for 100 tests (1)
Download data sheet
130-091-650
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| Anti-TNF-α-PE, human |
for 100 tests (1)
Download data sheet
130-091-651
|
| Anti-TNF-α-APC, human |
for 100 tests (1)
Download data sheet
130-091-649
|
| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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