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| Description |
| Interferon gamma (IFN-γ) is produced by cells that are involved in inflammatory immune responses. IFN-γ is predominantly released by memory and effector CD4+ and CD8+ T cells as well as by NK cells upon activation. |
| Applications |
| This Anti-IFN-γ antibody has been developed for intracellular staining of IFN-γ in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by solid-phase intracellular staining technology in combination with MACS® Separation. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| 45-15 | Mouse IgG1 |
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| Figure 1 |
| Human PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE. Cells were then analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-FITC staining. |
| Stimulated sample |
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| Unstimulated control |
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| Products |
| Anti-IFN-γ-FITC, human |
for 100 tests (1)
Download data sheet
130-091-641
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| Anti-IFN-γ-PE, human |
for 100 tests (1)
Download data sheet
130-091-653
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| Anti-IFN-γ-APC, human |
for 100 tests (1)
Download data sheet
130-091-640
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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