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| Mouse IgG1 - isotype control antibodies |
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| Description |
| The Mouse IgG1 isotype control antibody clone IS5-21F5 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on human cells or cell lines. Therefore, the antibody clone IS5-21F5 can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG1 fluorochrome-conjugated antibodies to human cells, for example via Fc receptors, or due to interactions of the fluorochrome with the cell surface. |
| Applications |
| Mouse IgG1 isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analysis of human cells. |
| Clone | Isotype |
| IS5-21F5 | Mouse IgG1 |
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| Figure 1 |
| Human PBMCs were stained with Mouse IgG1 antibodies conjugated to PE (A) or APC (B) and analyzed by flow cytometry. Cells stained with IgG1-Biotin (C) were stained with Anti-Biotin-APC in addition. |
| A |
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| B |
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| C |
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| Products |
| Mouse IgG1-FITC |
- For 100 tests (1)
Download data sheet
130-092-213
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| Mouse IgG1-PE |
- For 100 tests (1)
Download data sheet
130-092-212
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| Mouse IgG1-APC |
- For 100 tests (1)
Download data sheet
130-092-214
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| Mouse IgG1-Biotin |
- For 100 tests (2)
Download data sheet
130-093-018
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| (1) One test corresponds to fluorescent labling of up to 107 cells in a total volume of 100µL. |
| (2) One test corresponds to labling of up to 107 cells in a total volume of 100µL. |
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