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Mouse IgG2a - isotype control antibodies
Description
The Mouse IgG2a isotype control antibody clone S43.10 is specific for the hapten NP (4-hydroxy-3-nitro-phenyl) acetyl. This hapten is not expressed on cells or cell lines. Therefore, the antibody clone S43.10 can be used as a negative control to distinguish specific from non-specific binding of mouse IgG2a fluorochrome-conjugated antibodies to human cells, for example, via Fc receptors or due to interactions of the fluorochrome with the cell surface.
Applications
Mouse IgG2a isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analysis of human cells.
CloneIsotype
S43.10Mouse IgG2a
 
Figure 1
Human PBMCs were stained with Mouse IgG2a antibodies conjugated to FITC (A), PE (B), or APC (C), and analyzed by flow cytometry.
A
B
C
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Products
Mouse IgG2a-FITC
for 100 tests (1)
Download data sheet
130-091-837
Qty:
 

Mouse IgG2a-PE
for 100 tests (1)
Download data sheet
130-091-835
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Mouse IgG2a-APC
for 100 tests (1)
Download data sheet
130-091-836
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(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL.
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