CD137
Description This antibody recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4+ and CD8+ T cells, activated B cells, and natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule it is involved in the activation and survival of CD4+ or CD8+ T cells, and NK cells. Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8+ T cells, as CD137 is not expressed on resting CD8+ T cells and its expression is reliably induced after 24 hours of stimulation.1 In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.2 Applications The CD137 antibody is suitable for identification and enumeration of activated antigen-specific CD137+ T cells by flow cytometry or fluorescence microscopy. Other applications include the analysis of antigen-specific CD8+ T cells in combination with a MACS® Cytokine Secretion Assay – Detection Kit, or the evaluation of intracellular cytokine expression in activated antigen-specific CD8+ T cells by using the CD137 antibody in combination with antibodies against human cytokines. Cross-reactivity Rhesus monkey (Macaca mulatta) Cynomolgus monkey (Macaca fascicularis) Clone Isotype 4B4-1 Mouse IgG1		Figure 1 PBMCs of a CMV+ donor were stimulated overnight using PepTivator™ CMV pp65 or left untreated. Cells were stained with CD8-FITC and CD137-PE. Cell debris and dead cells were excluded from the analysis according to PI fluorescence. Stimulated sample Unstimulated control

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