|
|
 |
|
| Description |
The Anti-IgE antibody, clone MB10-5C4, detects the IgE isotype of human immunoglobulins.
IgE plays an important role in allergy. It is especially associated with type 1 hypersensitivity, where IgE shows high-affinity binding to Fc receptors expressed on the surface of mast cells. Antigen binding to Fc receptor–bound IgE upon re-exposure to specific allergens results in degranulation and the release of a variety of mediators, such as histamine and cytokines.
IgE has two main receptors: the high-affinity receptor FcεRI, which is expressed only on mast cells and basophils, and the low-affinity receptor FcεRII (CD23), which is expressed on B cells. |
| Applications |
| Identification and enumeration of human IgE+ cells by flow cytometry and fluorescence microscopy. Intracellular staining of IgE-producing cells and staining of Fc receptor–bound immunoglobulins |
| Clone | Isotype |
| MB10-5C4 | Mouse IgG1 |
| |
| Figure |
| Human PBMCs were stained with Anti-IgE antibodies conjugated to FITC (A), PE (B), APC (C), or Biotin (D) and with CD19 antibodies, and analyzed by flow cytometry. Cell stained with Anti-IgE-Biotin were stained with Anti-Biotin-APC in addition. |
| A |
 |
| B |
 |
| C |
 |
| D |
 |
|
|
|
|
| My favorites |
| Your current favorite list is empty. |
| Products |
| Anti-IgE-FITC, human |
- for 100 tests (1)
Download data sheet
130-093-065
|
| Anti-IgE-PE, human |
- for 100 tests (1)
Download data sheet
130-093-066
|
| Anti-IgE-APC, human |
- for 100 tests (1)
Download data sheet
130-093-067
|
| Anti-IgE-Biotin, human |
- for 100 tests (2)
Download data sheet
130-093-068
|
| Anti-IgE pure, human |
- 100 µg in 1 mL
Download data sheet
130-093-127
|
| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 µL |
|
|