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| Description |
The Anti-PSA-NCAM antibody recognizes an epitope on human, mouse, and rat polysialic acid (PSA) which, in vertebrates, is found linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56)1. PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue.Ā² In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ)3 and the dentate gyrus of the hippocampusā“.
PSA-NCAM is a marker for immature neuronal-committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons.3,5
Antibodies against PSA-NCAM have also been used to immunomagnetically isolate neuronal progenitors from postnatal mice and rat forebrain.6-8 |
| Applications |
| Identification and enumeration of human, mouse, rat PSA-NCAM+ cells by flow cytometry, fluorescence microscopy, or immunohistochemistry in conjunction with a conjugated secondary antibody. |
| Clone | Isotype |
| 2-2B | Mouse IgM |
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| Figure 1 |
| Mouse brain tissue postnatal day 1 was dissociated using the Neural Tissue Dissociation Kit (T). Brain cells were stained with Anti-PSA-NCAM-PE (A) or Anti-PSA-NCAM-APC (B). Cells were analyzed by flow cytometry. |
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| B |
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| Products |
| Anti-PSA-NCAM-PE, human, mouse, rat |
- for 100 tests (1)
Download data sheet
130-093-274
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ĀµL. |
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| MACS References |
| 1. Rougon, G. and Marshak, D. R. (1986) J. Biol. Chem. 261: 3396ā3401. |
| 2. Kiss, J. Z. and Muller, D. (2001) Rev. Neurosci. 12: 297ā310. |
| 3. Doetsch, F. et al. (1997) J. Neurosci. 17: 5046ā5061. |
| 4. Seki, T. (2002) J. Neurosci. Res. 70: 327ā334. |
| 5. Pennartz, S. et al. (2004) Mol. Cell. Neurosci. 25: 692ā706. |
| 6. Seidenfaden, R. et al. (2006) Mol. Cell. Neurosci. 32: 187ā98. |
| 7. Seidenfaden, R. et al. (2006) MACS&more 10(1): 4ā6. |
| 8. Marmur, R. et al. (1998) J. Neurosci. 18: 9800ā9811. |
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