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| Description |
The Anti-Slan (M-DC8) antibody (clone DD-1) recognizes Slan (6-Sulfo LacNAc), which is a carbohydrate modification of P-selectin glycoprotein ligand-1 (PSGL-1). This antigen is characteristically expressed on a new subset of PBMCs with features closely related to CD16+CD14low monocytes. Slan (M-DC8)+ cells constitute 0.5–2% of all PBMCs with similar frequencies among mononuclear cells from cord blood. |
| Applications |
| The antibody can be used to identify and enumerate Slan (M-DC8)+ cells by flow cytometry or fluorescence microscopy. This antibody is suited to evaluate MACS® Separations. For further details, please refer to the datasheet of the separation reagent. |
| Clone | Isotype |
| DD-1 | Mouse IgM |
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| Figure 1 |
| Human PBMCs were stained with Anti-Slan (M-DC8) antibodies conjugated to FITC (A), PE (B), or APC (C) as well as with CD14 antibodies and analyzed by flow cytometry. Cells stained with Anti-Slan (M-DC8)-Biotin (D) were stained with Anti-Biotin-APC in addition. |
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| B |
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| C |
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| D |
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| Products |
| Anti-Slan (M-DC8)-FITC, human |
For 100 tests (1) Download data sheet 130-093-027
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| Anti-Slan (M-DC8)-PE, human |
For 100 tests (1) Download data sheet 130-093-029
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| Anti-Slan (M-DC8)-APC, human |
For 100 tests (1) Download data sheet 130-093-031
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| Anti-Slan (M-DC8)-Biotin, human |
For 100 tests (2) Download data sheet 130-093-033
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 µL. |
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