Anti-FoxP3
Description The Anti-FoxP3 antibody specifically recognizes the forkhead/winged-helix transcriptional regulator FoxP3, also known as forkhead box P3, scurfin, and JM2. It is expressed predominantly in Tregs and is a major marker and functional regulator of Treg cell development and function.1,2 Mutations in the FoxP3 gene are linked to the autoimmune manifestations observed in the Scurfy mouse and humans with immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome.3,4 Studies in mice have shown that FoxP3-deficient animals lack Treg cells, whereas overexpression of the FoxP3 protein leads to profound immune suppression. The FoxP3 Staining Buffer Set is optimized for intracellular staining of FoxP3 with clone 3G3. Applications The antibody is suitable for intracellular staining of FoxP3 in human, mouse, and non-human primate cells. Cross-reactivity Rhesus monkey (Macaca mulatta) Cynomolgus monkey (Macaca fascicularis) Clone Isotype 3G3 Mouse IgG1		Figure 1 Human PBMCs were stained with CD4-FITC and CD25-APC or CD25-PE, respectively. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry. A B

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