Anti-Ig λ Light Chain
Description Immunoglobulins produced by B cells are composed of two heavy and two light chains, connected by disulfide bonds. Differences in the composition of the heavy chain constant regions determine the isotype of the antibody. The light chains of all isotypes are either of κ or λ subtype. Clone IS7-24C7 detects human Ig light chains of the λ subtype. Heavy chains and light chains of the κ subtype are not detected. Applications Identification and enumeration of human immunoglobulins with λ light chains by flow cytometry or fluorescence microscopy. Cross-reactivity Rhesus monkey (Macaca mulatta) Cynomolgus monkey (Macaca fascicularis) Clone Isotype IS7-24C7 Mouse IgG1		Figure 1 Human PBMCs were stained with Anti-Igλ light chain antibodies coupled to FITC (a), PE (b) or APC (c) and analyzed by flow cytometry. Cells stained with Anti-Igλ Light Chain-Biotin were stained with Anti-Biotin-PE in addition. a b c d Figure 2 Human PBMCs were fixed, permeabilized and intracellularly stained with Anti-Ig λ light chain antibodies coupled to PE (a) or APC (b). Cells were analyzed by flow cytometry. a b