CD209 (DC-SIGN)
Description Clone DCN47.5 recognizes CD209 (Dendritic Cell Specific ICAM-3 Grabbing Non-integrin, DC-SIGN), a type II c-type lectin expressed in dermal and mucous tissue, lymphoid tissue such as tonsil, lymph node, spleen, and on monocyte-derived DCs (MoDCs). It is not expressed on DC subsets in peripheral blood, except for a subpopulation of CD14+ cells with a DC-like phenotype. DC-SIGN acts as an adhesion receptor to facilitate interactions between DCs and T cells or DCs and endothelial cells. In addition, it serves as an antigen receptor mediating internalization of ligands for antigen presentation, binding virus such as HIV, HCMV, or Ebola as well as parasites, bacteria, or yeast. HIV-1 uses this feature of DC-SIGN for efficient in -trans infection of CD4+ T cells. Applications The antibody is suitable for identification and enumeration of CD209 (DC-SIGN)+ cells by flow cytometry or fluorescence microscopy. Clone Isotype DCN47.5 Mouse IgG1		Figure 1 Human monocyte-derived dendritic cells (Mo-DCs) were stained with CD209 (DC-SIGN) antibodies conjugated to FITC (A), PE (B), or APC (C) and analyzed by flow cytometry. Cells stained with CD209 (DC-SIGN)-Biotin (D) were stained with Anti-Biotin-APC in addition. Black line represents staining with CD209 (DC-SIGN) antibodies, gray line represents isotype control. A B C D

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