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| Description |
Clone DX2 recognizes the human CD95 antigen. CD95, also termed Fas and Apo-1, is a member of the tumor necrosis factor receptor superfamily (TNFR) and is found on the surface of many normal and neoplastically transformed cells. Its ligand, CD95L (FasL/Apo-1L), is able to induce apoptosis in CD95-expressing cells upon binding1. CD95 and CD95L are up-regulated on lymphocytes upon activation and are known to play a key role in the regulation of an inflammatory response: Juxtocrine “fratricide” of neighbouring lymphocytes via mutual CD95 and CD95L expression helps to terminate immune responses2, while apoptosis of pro-inflammatory cells via CD95 helps maintain immune privilege in sites such as the eye, where CD95L is found to be expressed in the retina and cornea3. Cross-linking of CD95 receptors by DX2 monoclonal antibody has been described to induce apoptosis in certain target cells.4
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| Applications |
| Identification and enumeration of CD95+ cells by flow cytometry and fluorescence microscopy. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Figure 1 |
| Human peripheral blood CD4+ cells were stained with CD95 antibodies conjugated to FITC, as well as CD45RO-PE, and analyzed by flow cytometry. |
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| References |
| 1. Itoh, N. et al. (1991) Cell 66: 233–243. |
| 2. Lynch, D. et al. (1995) Immunol. Today 16: 569–574. |
| 3. Griffith, T. et al. (1995) Science 270: 1189–1192. |
| 4. Komada, Y. et al. (1999) Hybridoma 18: 391–398. |
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