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CD4 (VIT4)
Description
The CD4 antibody recognizes the human CD4 antigen which is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC Class II antigen.
The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
The VioBlue-conjugated CD4 antibody requires the use of a violet laser.
Applications
The antibody can be used for the identification and enumeration of CD4+ cells by flow cytometry or fluorescence microscopy and is suitable for MACS® Control applications. For further details see the datasheet of the separation reagent.
CD4 antibodies can be used for evaluating the transfection efficiency of cells transfected with pMACS 4.1 or pMACS 4-IRES when using the MACSelect™ 4 Transfected Cell Selection Kit.
The antibody is suitable for staining of fixed cells.
Clone Isotype
VIT4Mouse IgG2a
 
Figure 1
Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies conjugated to FITC (a), PE (b), APC (c), or VioBlue (d) and analyzed by flow cytometry.
a
b
c
d
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Products
CD4 (VIT4)-FITC, human
- for 100 tests (1)
Download data sheet
130-092-358
Qty:
 

CD4 (VIT4)-PE, human
- for 100 tests (1)
Download data sheet
130-092-373
Qty:
 

CD4 (VIT4)-APC, human
- for 100 tests (1)
Download data sheet
130-092-374
Qty:
 

CD4 (VIT4)-VioBlue, human
Download data sheet
130-094-153
Qty:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL.
Related products
MACSelect™ 4 System
CD4 MicroBeads
Whole Blood CD4 MicroBeads
CD4+ T Cell Isolation Kit II, human
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