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MACSQuant® Analyzer
navigationAntibodies
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for human cellsfor human cellsfor human cells
AntibodiesAntibodiesAntibodiesAntibodies
Cytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodies
Isotype control antibodiesIsotype control antibodiesIsotype control antibodiesIsotype control antibodies
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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for mouse cellsfor mouse cellsfor mouse cells
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for rat cellsfor rat cellsfor rat cells
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for indirect stainingfor indirect stainingfor indirect staining
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Cytokine detection
Kits for cell detection and analysis
Tumor cell analysis
Immunofluorescent amplification (FASER Kits)
Inside Stain Kit
Staining of apoptotic and dead cells
Compensation reagents
Antibody biotinylation
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CD4 (VIT4)
Description
The CD4 antibody recognizes the human CD4 antigen which is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC Class II antigen.
The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
The VioBlue-conjugated CD4 antibody requires the use of a violet laser.
Applications
The antibody can be used for the identification and enumeration of CD4+ cells by flow cytometry or fluorescence microscopy and is suitable for MACS® Control applications. For further details see the datasheet of the separation reagent.
CD4 antibodies can be used for evaluating the transfection efficiency of cells transfected with pMACS 4.1 or pMACS 4-IRES when using the MACSelect™ 4 Transfected Cell Selection Kit.
The antibody is suitable for staining of fixed cells.
Clone Isotype
VIT4Mouse IgG2a
 
Figure 1
Human PBMCs were stained with CD4 (VIT4) antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (E), and analyzed by flow cytometry.
A
B
C
D
E
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Products
CD4 (VIT4)-FITC, human
- for 100 tests (1)
Download datasheet
130-092-358
Qty.:
 

CD4 (VIT4)-PE, human
- for 100 tests (1)
Download datasheet
130-092-373
Qty.:
 

CD4 (VIT4)-APC, human
- for 100 tests (1)
Download datasheet
130-092-374
Qty.:
 

CD4 (VIT4)-VioBlue, human
- for 100 tests (1)
Download datasheet
130-094-153
Qty.:
 

CD4 (VIT4)-PerCP, human
- for 100 tests (1)
Download datasheet
130-094-963
Qty.:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL.
Related products
MACSelect™ 4 System
CD4 MicroBeads
Whole Blood CD4 MicroBeads
CD4+ T Cell Isolation Kit II, human
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