CD203c
Description Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils, mast cells and their precursors, and has been described as specific for this lineage.1 Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate2. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g. in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g. in flow cytometric basophil activation tests of the peripheral blood3–5. Applications CD203c antibodies can be used to identify and enumerate human basophils in peripheral blood or bone marrow by flow cytometry and microscopy. This antibody is suitable MACS® Control applications, for example, when using the Basophil Isolation Kit II, human. If preferred, cells may be counterstained with CD123 antibodies and identified as CD203c+CD123bright cells. Clone Isotype FR3-16A11 Mouse IgG1		Figure 1 Human PBMCs were stained with CD203c-PE and CD123-APC (A), CD203c-APC and CD123-PE (B), or CD203c-Biotin, Anti-Biotin-PE, and CD123-APC (C). Cells were then analyzed by flow cytometry. A: PBMCs stained with CD203c-PE and CD123-APC. B: PBMCs stained with CD203c-APC and CD123-PE. C: PBMCs stained with CD203c-Biotin, Anti-Biotin-PE, and CD123-APC.

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