| Description |
The CD133/1 (W6B3C1) antibody recognizes the glycosylated epitope 1 of the human CD133 antigen, a 120 kDa 5-transÂmembrane domain glycoprotein1,2. Four monoclonal antibodies recognizing the CD133 antigen are available: clone AC133 (mouse IgG1), 293C3 (mouse IgG2b), W6B3C1 (mouse IgG1) and AC141 (mouse IgG1). These antibodies recognize two different epitopes CD133/1 (clone AC133 and W6B3C1) and CD133/2 (clone 293C3 and clone AC141), respectively.
CD133 is found to be expressed not only on hematopoietic and nonhematopoietic stem and progenitor cells in bone marrow, cord blood, and peripheral blood but also on stem and progenitor cells in different tissues such as brain3,4, prostatic tissue5, muscle6, or stroma of the cornea7. |
| Applications |
| The antibody CD133 (W6B3C1) has been optimized for the evaluation of CD133 expression in human tissues by Western blot analysis. |
| Clone | Isotype |
| W6B3C1 | Mouse lgG1 |
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| Figure 1 |
| Western blot analysis of 2Ă—105 PBMCs (lane 1) spiked with 10% (lane 2), 5% (lane 3), 1% (lane 4), 0.5% (lane 5), and 0.2% (lane 6) WERI-Rb-1 cells, a CD133-expressing cell line. M = Marker. |
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| Figure 2 |
| Protein extracts of human hippocampus (Lane 2), human fetal brain (Lane 3) and Weri-Rb-1 cells (Lane 5) were blotted onto a PVDF membrane and probed with CD133/1 (W6B3C1) pure antibody. Lane 4 is empty. Goat anti-mouse IgG-HRP was applied at a dilution of 1:10,000 before bands were developed using ECL Plus™ chemiluminescent detection reagent. The molecular weight marker (Lane 1) indicates CD133 reactivity at approximately 120 kDa in Lanes 3 and 5. |
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