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| Description |
| Clone AC133 recognizes the glycosylated epitope 1 of the human CD133 antigen (CD133/1). The CD133 antigen is expressed on stem cells with hematopoietic and nonhematopoietic differentiation potential. Four monoclonal antibodies recognizing the CD133 antigen are available: clone AC133 (mouse IgG1), 293C3 (mouse IgG2b), W6B3C1 (mouse IgG1) and AC141 (mouse IgG1). These antibodies recognize two different epitopes CD133/1 (clone AC133 and W6B3C1) and CD133/2 (clone 293C3 and clone AC141), respectively. |
| Applications |
Clone AC133 is recommended for the identification and enumeration of CD133+ cells from peripheral blood, bone marrow, cord blood, or other tissues. The antibody can also be used to detect endothelial progenitor cells (EPCs).
CD133/1 can also be used in immunohistochemical applications; a protocol for the staining of paraffin-embedded tissue sections is available here.
It is not recommended for staining of CD133+ cells isolated with the CD133 MicroBead Kit. |
| Clone | Isotype |
| AC133 | Mouse IgG1 |
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| Figure 1 |
| Staining of non-mobilized PBMCs with CD133/1-PE and CD45-FITC. |
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| Figure 2 |
| Staining of non-mobilized PBMCs with CD133/1-APC and CD34-FITC. |
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| Products |
| CD133/1 (AC133)-PE, human |
for 100 tests (1)
Download data sheet
130-080-801
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| CD133/1 (AC133)-APC, human |
for 100 tests (1)
Download data sheet
130-090-826
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| CD133/1 (AC133)-Biotin, human |
for 100 tests (2)
Download data sheet
130-090-664
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| CD133/1 (AC133) pure, human |
50 ÎĽg in 1 mL
Download data sheet
130-090-422
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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