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| Description |
| The Anti-PTK7 (CCK-4) antibody (clone 188B) recognizes human protein tyrosine kinase-7 (PTK7). PTK7, also known as colon carcinoma kinase-4 (CCK-4), is a receptor protein tyrosine kinase (RPTK)-like molecule that contains a catalytically inactive tyrosine kinase domain.1,2 PTK7 was identified to be important for neural development as a regulator of planar cell polarity, being required for convergent extension movement and neural tube closure.3 Using the Anti-PTK7 (CCK-4) antibody, PTK7 expression was recently analyzed on blood and bone marrow cells. PTK7 was shown to be expressed on plasmacytoid dendritic cells, CD141 (BDCA-3)high type-2 myeloid dendritic cells, and CD34+ hematopoietic progenitor cells (HPCs). PTK7 was detected on early (CD34+ CD133+) and late (CD34+ CD133–) HPCs. In tonsils, PTK7 was also found on some T cells. |
| Applications |
Identification of PTK7+ cells by fluorescence microscopy and flow cytomety. The antibody is suitable for staining of fresh or formaldehyde-fixed cells in suspension. Staining with Anti-PTK7 (CCK‑4) antibodies is recommended for flow cytometric evaluation if PTK7+ cells are isolated using the
Anti-PTK7 (CCK-4) MicroBead Kit. |
| Clone | Isotype |
| 188B | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with Anti-PTK7-APC and CD303 (BDCA-2)-FITC, CD141 (BDCA-3)-PE, or CD34-FITC, respectively. Cells were analyzed by flow cytometry. Plasmacytoid dendritic cells (PDCs) are identified by CD303 (BDCA-2), type-2 myeloid dendritic cells (MDC2s) by CD141 (BDCA-3), and hematopoietic stem cells (HPCs) by CD34 expression. |
| Identification of PTK7+ CD141 (BDCA-3)high MDC2s |
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| Identification of PTK7+ CD34+ HPCs |
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| Identification of PTK7+ CD303 (BDCA-2)+ PDCs |
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| Products |
| Anti-PTK7 (CCK-4)-PE, human |
for 100 tests (1)
Download data sheet
130-091-364
|
| Anti-PTK7 (CCK-4)-APC, human |
for 100 tests (1)
Download data sheet
130-091-366
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| Anti-PTK7 (CCK-4)-Biotin, human |
for 100 tests (2)
Download data sheet
130-091-365
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| Anti-PTK7 (CCK-4) pure, human |
100 ÎĽg in 1 mL
Download data sheet
130-091-578
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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| MACS References |
| 1. Mossie et al. (1995) Oncogene 11: 2179-2184.[8494] |
| 2. Park et al. (1996) J. Biochem. 119: 235-239.[8495] |
| 3. Lu et al. (2004) Nature 430: 93-98.[8297] |
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