CD117 (A3C6E2)
Description The CD117-specific monoclonal antibody (mAb) A3C6E2 recognizes the human CD117 antigen. Clone A3C6E2 recognizes an epitope which is different from the epitope recognized by the CD117 mAb clone AC126. CD117 (also known as c-kit or SCF receptor) is a 145 kDa cell surface glycoprotein with tyrosine kinase activity. This molecule is suggested to be involved in signaling, activation, and proliferation of cells. The CD117 antigen is expressed on about 1-3% of peripheral blood mononuclear cells (PBMCs) and cord blood cells and on up to 10% of bone marrow cells. Around 25% of CD117 + cells were found to coexpress CD133 and CD34. CD117 is further expressed on basophils, myeloid dendritic cells, TCRα/β + T cells, CD19 + B cells, and CD56 + NK cells 1 , as well as on mast cells, melanocytes, and AML (acute myeloid leukemia) blasts 2 . Applications Clone A3C6E2 is recommended for MACS® Control applications following the use of the CD117 MicroBead Kit, human as it recognizes a different epitope to that of the MicroBead-coupled CD117 antibody. It should be noted that clone A3C6E2 may potentially block the binding of SCF to CD117. CD117 (A3C6E2) can otherwise be used for the identifcation and enumeration of CD117+ cells by flow cytometry or fluorescence microscopy. Clone Isotype A3C6E2 Mouse IgG1		Figure 1 Human cord blood mononuclear cells (CB-MNCs) were stained with CD117 antibodies conjugated to PE (A) or APC (B) and CD133 antibodies conjugated to APC (A) or PE (B), and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. A B

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