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CD45
Description
The CD45 antibody recognizes the human CD45 antigen, a tyrosine phosphatase also known as the leukocyte common antigen (LCA). CD45 is present on all human cells of hematopoietic origin, except erythroid cells, platelets, and their precursor cells. The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell.
The CD45 antibody recognizes a common epitope of all CD45 isoforms.
The VioBlue-conjugated CD45 antibody requires the use of a violet laser.
Applications
The CD45 antibody can be used to identify and enumerate CD45+ cells by flow cytometry or fluorescence microscopy.
This antibody is suitable for MACSĀ® Control applications. For further details, please refer to the data sheet of the separation reagent.
CloneIsotype
5B1Mouse IgG2a
 
Figure 1
Human peripheral blood leukocytes were stained with CD45-FITC (A). Human PBMCs were stained with CD45-PE (B), CD45-APC (C), or CD45-VioBlue (D). Cells were analyzed by flow cytometry.
A
B
C
D
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Products
CD45-FITC, human
for 100 tests (1)
Download data sheet
130-080-202
Qty:
 

CD45-PE, human
for 100 tests (1)
Download data sheet
130-080-201
Qty:
 

CD45-APC, human
for 100 tests (1)
Download data sheet
130-091-230
Qty:
 

CD45-VioBlue, human
for 100 tests (1)
Download data sheet
130-092-880
Qty:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL.
Related products
Mouse IgG2a - isotype control antibodies
CD45 MicroBeads, human (#130-045-801)
Whole Blood CD45 MicroBeads, human (#130-090-872)
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