CD45
Description The CD45 antibody recognizes the human CD45 antigen, a tyrosine phosphatase also known as the leukocyte common antigen (LCA). CD45 is present on all human cells of hematopoietic origin, except erythroid cells, platelets, and their precursor cells. The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms. The VioBlue-conjugated CD45 antibody requires the use of a violet laser. Applications The CD45 antibody can be used to identify and enumerate CD45+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent. Clone Isotype 5B1 Mouse IgG2a		Figure 1 Human peripheral blood leukocytes were stained with CD45-FITC (A). Human PBMCs were stained with CD45-PE (B), CD45-APC (C), or CD45-VioBlue (D). Cells were analyzed by flow cytometry. A B C D