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MACSQuant® Analyzer
navigationAntibodies
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Biotec
for human cellsfor human cellsfor human cells
AntibodiesAntibodiesAntibodiesAntibodies
Cytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodies
Isotype control antibodiesIsotype control antibodiesIsotype control antibodiesIsotype control antibodies
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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Biotec
for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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Biotec
for mouse cellsfor mouse cellsfor mouse cells
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Biotec
for rat cellsfor rat cellsfor rat cells
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Biotec
for indirect stainingfor indirect stainingfor indirect staining
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Biotec
Cytokine detection
Kits for cell detection and analysis
Tumor cell analysis
Immunofluorescent amplification (FASER Kits)
Inside Stain Kit
Staining of apoptotic and dead cells
Compensation reagents
Antibody biotinylation
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CD25
Description
The CD25 antibody recognizes the human CD25 antigen, a 55 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4+ regulatory T cells.
The CD25 antigen contains three epitope regions called A, B, and C. This CD25 antibody recognizes epitope region B.
Applications
The CD25 antibody can be used to identify and enumerate CD25+ cells by flow cytometry or fluorescence microscopy.
This antibody is ideally suited to MACS® Control applications after use of CD25 MicroBeads as the CD25 antibody recognizes a different epitope (epitope B) than the CD25 MicroBeads (epitope A). For further details, please refer to the data sheet of the separation reagent.
Cross-reactivity
  • Rhesus monkey (Macaca mulatta)
  • Cynomolgus monkey (Macaca fascicularis)
Clone Isotype
4E3Mouse IgG2b
 
Figure 1
CD4+ human cord blood lymphocytes were isolated with the CD4+ T Cell Isolation Kit II, stained with CD25-PE, and analyzed by flow cytometry.
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Products
CD25-PE, human
- for 100 tests (1)
Download datasheet
130-091-024
Qty.:
 

CD25-APC, human
- for 100 tests (1)
Download datasheet
130-092-858
Qty.:
 

CD25-Biotin, human
- for 100 tests (2)
Download datasheet
130-091-235
Qty.:
 

(1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL.
(2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL.
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