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| Description |
The CD25 antibody recognizes the human CD25 antigen, a 55 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4+ regulatory T cells.
The CD25 antigen contains three epitope regions called A, B, and C. This CD25 antibody recognizes epitope region B. |
| Applications |
The CD25 antibody can be used to identify and enumerate CD25+ cells by flow cytometry or fluorescence microscopy.
This antibody is ideally suited to MACS® Control applications after use of CD25 MicroBeads as the CD25 antibody recognizes a different epitope (epitope B) than the CD25 MicroBeads (epitope A). For further details, please refer to the data sheet of the separation reagent. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| 4E3 | Mouse IgG2b |
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| Figure 1 |
| CD4+ human cord blood lymphocytes were isolated with the CD4+ T Cell Isolation Kit II, stained with CD25-PE, and analyzed by flow cytometry. |
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| Products |
| CD25-PE, human |
- for 100 tests (1)
Download datasheet
130-091-024
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| CD25-APC, human |
- for 100 tests (1)
Download datasheet
130-092-858
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| CD25-Biotin, human |
- for 100 tests (2)
Download datasheet
130-091-235
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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