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| CD304 (BDCA-4/Neuropilin-1) |
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| Description |
| Clone AD5-17F6 recognizes the human CD304 (BDCA-4/Neuropilin-1) antigen. Like CD303 (BDCA-2), the CD304 (BDCA-4/Neuropilin-1) antigen1 is specifically expressed on human plasmacytoid dendritic cells in blood1–3 and bone marrow4. Unlike CD303 (BDCA-2), expression of CD304 (BDCA-4/Neuropilin-1) is up-regulated on plasmacytoid dendritic cells and induced on myeloid cells upon culturing.4 In inflamed tonsils, CD304 (BDCA-4/Neuropilin-1) expression, apart from plasmacytoid dendritic cells, is also detected primarily on follicular B helper memory T cells.4 CD304 (BDCA-4) is identical to neuropilin-1 (NP-1),4 a neuronal receptor, which is known to be expressed on numerous nonhematopoietic cell types, including neurons, endothelial, and tumor cells. CD1a+ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells are CD304 (BDCA-4/Neuropilin-1)+ but CD303 (BDCA-2)–.1 |
| Applications |
| Direct identification of CD304 (BDCA-4/Neuropilin-1)+ plasmacytoid dendritic cells in blood and bone marrow by immunofluorescent staining and flow cytometric analysis.1,4 The antibody is suitable for staining of fresh or formaldehyde-fixed cells in suspension. Staining with CD304 (BDCA-4) antibodies is recommended for flow cytometric evaluation if plasmacytoid dendritic cells are isolated using the CD303 (BDCA-2) MicroBead Kit. |
| Clone | Isotype |
| AD5-17F6 | Mouse IgG1 |
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| Figure 1 |
| Peripheral blood leukocytes were stained with CD304 (BDCA-4/Neuropilin-1)-PE and CD123-APC. Note that CD304 (BDCA-4) is exclusively expressed on plasmacytoid dendritic cells. |
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| Products |
| CD304 (BDCA-4/Neuropilin-1)-PE, human |
for 100 tests (1)
Download data sheet
130-090-533
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| CD304 (BDCA-4/Neuropilin-1)-APC, human |
for 100 tests (1)
Download data sheet
130-090-900
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| CD304 (BDCA-4/Neuropilin-1)-Biotin, human |
for 100 tests (2)
Download data sheet
130-090-748
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| CD304 (BDCA-4/Neuropilin-1) pure, human |
100 ÎĽg in 1 mL
Download data sheet
130-090-693
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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| MACS References |
| 1. Dzionek et al. (2000) J. Immunol. 165: 6037–6046.[898] |
| 2. Grabbe et al. (2000) Immunol. Today 21: 431–433.[899] |
| 3. Dzionek et al. (2001) J. Exp. Med. 194: 1823–1834.[1264] |
| 4. Dzionek et al. (2002) Human Immunol. 36: 1133–1148.[2423] |
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