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| Description |
The clone AD5-8E7 recognizes human CD1c, also known as BDCA-11. CD1c (BDCA-1) is expressed on a major subpopulation of human myeloid dendritic cells (about 0.3% of blood leukocytes). Blood CD1c (BDCA-1)+ myeloid dendritic cells are CD11chigh, CD123low, CD4+, Lin–, CD45RO+, CD2+, CD16–, CD141 (BDCA-3)low, CD303 (BDCA-2)–, and CD304 (BDCA-4/Neuropilin-1)–.1–4 They express myeloid markers (CD13, CD33) as well as Fc receptors (CD32, CD64, FcεRI) and are of monocytoid appearance.1 A minor proportion of CD1c (BDCA-1)+ myeloid dendritic cells expresses CD14 and CD11b. In blood, CD1c (BDCA-1) is also expressed on a subpopulation of CD19+ small resting B lymphocytes. CD1c (BDCA-1) expression has also been detected on cortical thymocytes, on Langerhans cells7, and on CD1a+ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells. CD1c (BDCA-1)+ myeloid dendritic cells have been designated as type-1 myeloid dendritic cells (MDC1s).
The CD1c antigen is a member of the CD1 family of proteins that are structurally related to MHC Class I proteins and mediate the presentation of non-peptide antigens to T cells.5
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| Applications |
| Direct identification of CD1c (BDCA-1)+ myeloid dendritic cells in blood by immunofluorescent staining and flow cytometric analysis1 or tissue sections and immunocytochemical staining6. The antibody is suitable for staining of fresh or formaldehyde-fixed cells in suspension, and for staining of air-dried, acetone-fixed cryosections, for example. CD1c (BDCA-1) antibodies were used for quantitation and characterization of dendritic cell subsets in blood3 or for localization of myeloid dendritic cells in lymphoid and non-lymphoid tissue6,7. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
- Pigtail monkey (Macaca nemestrina)
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| Clone | Isotype |
| AD5-8E7 | Mouse IgG2a |
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| Figure 1 |
| PBMCs were stained with CD1c (BDCA-1) and CD19 (A). Apart from type-1 myeloid dendritic cells (MDC1s), CD1c (BDCA-1) is also expressed on a subset of B cells. For this reason, B cells are excluded from the analysis according to expression of CD19 and further analysis is restricted to CD19– cells (red colored gate). Thereafter, CD1c (BDCA-1) is specifically expressed on HLA-DR+ (B), CD123dim (C), CD11c+ (D), and CD14+/– (E) cells, i.e. MDC1s. Note that some CD1c (BDCA-1)+ MDC1s express CD14. |
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| E |
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| Products |
| CD1c (BDCA-1)-FITC, human |
- for 100 tests (1)
Download data sheet
130-090-507
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| CD1c (BDCA-1)-PE, human |
- for 100 tests (1)
Download data sheet
130-090-508
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| CD1c (BDCA-1)-APC, human |
- for 100 tests (1)
Download data sheet
130-090-903
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| CD1c (BDCA-1)-Biotin, human |
- for 100 tests (2)
Download data sheet
130-090-692
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| CD1c (BDCA-1) pure, human |
- 100 ÎĽg in 1 mL
Download data sheet
130-090-695
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 ÎĽL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 ÎĽL. |
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| References |
| 1. Dzionek et al. (2000) J. Immunol. 165: 6037–6046.[898] |
| 2. Grabbe et al. (2000) Immunol. Today 21: 431–433.[899] |
| 3. MacDonald et al. (2002) Blood 100: 4512–4520.[2439] |
| 4. Clark et al. (2003) Transplant. 75: 221–225.[2873] |
| 5. Brigl and Brenner (2004) Annu. Rev. Immunol. 22: 817–890.[8493] |
| 6. Lebre, CM (2003) Doctoral dissertation, University of Amsterdam, Netherlands. |
| 7. Peiser et al. (2003) J. Immunol. Methods 279: 41–53.[4298] |
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