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| Description |
| Clone MB15-18C9 recognizes the CD127 antigen, which is the α-chain of the IL-7 receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).1 CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.2,3 On regulatory T cells CD127 is absent4 and its expression is inversely correlated with FoxP3 expression and suppressive function.5,6 CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.1 |
| Applications |
- Analysis of human regulatory T cells by using, for example, CD4-FITC, CD25-PE, and CD127-APC monoclonal antibodies.
- Identification of memory CD8+ T cell precursors within an effector T cell population.
- Evaluation of MACS® Separations by flow cytometry or fluorescence microscopy.
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| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| MB15-18C9 | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with CD127 antibodies conjugated to FITC (A), PE (B), or APC (C), as well as with CD4-PE or CD4‑APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD127-Biotin (D) were stained with Anti‑Biotin-PE and CD4-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| B |
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| C |
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| D |
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| Products |
| CD127-FITC, human |
- for 100 tests (1)
Download datasheet
130-094-888
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| CD127-PE, human |
- for 100 tests (1)
Download datasheet
130-094-889
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| CD127-APC, human |
- for 100 tests (1)
Download datasheet
130-094-890
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| CD127-Biotin, human |
- for 100 tests (2)
Download datasheet
130-094-891
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| CD127 pure, human |
- 100 μg in 1 mL
Download datasheet
130-094-942
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 µL. |
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| References |
| 1. Fry and Mackall (2002) Blood 99: 3892–3904. |
| 2. Sudo et al. (1993) J. Immunol. 90: 9125–9129. |
| 3. Armitage et al. (1991) Adv. Exp. Med. Biol. 292: 121–130. |
| 4. Cupedo et al. (2005) Eur. J. Immunol. 35: 383–390. |
| 5. Seddiki et al. (2006) J. Exp. Med. 203: 1693–1700. |
| 6. Liu et al. (2006) J. Exp. Med. 203: 1701–1711. |
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