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| CytoStim, non-human primate |
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| Description |
| CytoStim has been developed for rapid and efficient restimulation of non-human primate effector/memory T cells. |
| Details |
Background information
CytoStim causes activation of T cells by binding to the T cell receptor (TCR) and cross-linking it to a major histocompatibility complex molecule of an antigen–presenting cell. CytoStim is an antibody–based reagent that acts similarly to a superantigen but independently of certain Vβ domains of the TCR. Upon incubation with CytoStim, CD4+ and CD8+ cells start to secrete effector cytokines or up-regulate activation markers on their cell surface within a few hours. CytoStim is suitable for rapid stimulation of CD4+ and CD8+ T cells in fresh PBMCs, whole blood, or other leukocyte-containing single-cell suspensions from tissues. It can be used instead of SEB as a positive control in antigen-specific T cell stimulation assays or intracellular cytokine staining experiments to detect cytokine or activation marker expression. |
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| Figure 1 |
| PBMCs from rhesus monkey were incubated with CytoStim for 4 h and intracellulary stained with Anti‑IFN-γ-PE, CD4-APC and CD8-FITC (A, B) or with Anti‑IL‑17-PE, CD4‑APC, and CD8-FITC (C, D), respectively, using the Inside Stain Kit. |
| Stimulated sample |
| A: IFN-γ |
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| B: IFN-γ |
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| C: IL-17 |
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| D: IL-17 |
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| Unstimulated control |
| A: IFN-γ |
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| B: IFN-γ |
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| C: IL-17 |
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| D: IL-17 |
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